Sanger Sequencing
At Decipher Genomics & Research, we provide industry-leading Sanger Sequencing services using the ABI 3730xl DNA Analyzer paired with BigDye™ Terminator v3.1 chemistry. Recognized globally as the gold standard for confirmatory and targeted sequencing, this platform offers unparalleled precision for confirming short-read sequences, validating constructs, and advancing molecular diagnostics.
Whether you’re validating cloned constructs, sequencing direct PCR products, or analysing microbial cultures, our platform ensures exceptional base-calling accuracy, rapid turnaround, and flexible input types.
ABI 3730xl DNA Analyzer
What We Offer
- High-quality reads for targeted and confirmatory sequencing
- Flexible sample input options including crude and purified DNA, PCR products, and plasmids
- ISO-compliant processes and transparent, traceable workflows
Accepted Sample Types
- Direct PCR products
- Genomic DNA (gDNA)
- Plasmid DNA
- Purified or crude samples from tissues or non-pathogenic microbial cultures
- Nested PCR and cDNA
Our Quality-Driven Workflow
- Online Submission: Streamlined portal with barcoded tracking
- Sample Check-in: Physical and data QC by trained lab technicians
- Molecular QC: Assessment of DNA integrity, yield, and purity
- Sequencing Run: High-throughput analysis on ABI 3730xl platform
- Data Delivery: Base-called FASTA file, electropherogram, and trace file
Service Categories
| Service Type | Description | Typical Read Length |
| Single Pass Sequencing | DNA/plasmid isolation + one-direction read | Up to 800 bp |
| Bi-directional Sequencing | Both strand reads for enhanced fidelity | Up to 1,500 bp |
| Primer Walking (Single Direction) | Custom primer-based walk across template | 750–1,000 bp |
| Primer Walking (Bi-Directional) | Full duplex mapping for extended templates | 750–1,000 bp per strand |
Downloads
Seq – Plate Format Order form
Seq -Tube Order Form
Microbial Identification Order
Unraveling DNA with Unmatched Clarity — The Sanger Standard, Perfected.
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